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General description
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Application
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
to wash cells for protein isolation
Dulbecco′s Phosphate Buffered Saline has been used:
as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR[1]
to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells[2]
to wash cells for protein isolation[2]
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
provides a buffering system to maintain the medium within the physiological pH range
