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General description
For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.
Application
Glasgow Minimum Essential Medium has been used to culture:
Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
murine E14 Tg2a embryonic stem (mES) cells
transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins
Preparation Note
Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.
