General description
Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.[1]
Application
Discovery RP-AmideC16 HPLC Column may be used for the separation of:
Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).[1]
Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).[2]
Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.[3]
Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.[4]
Discovery® RP-AmideC16 HPLC column may be used for the separation of:
Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).[5]
Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.[6]
Hydrophilic bases by LC with principal component analysis (PCA).[7]
2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.[8]
Features and Benefits
Excellent retention and resolution of polar compounds
Unique selectivity compared to C18
Excellent peak shape and efficiency
Less hydrophobic than C18 columns
Compatible with 100% aqueous mobile phases
Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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