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General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Application
A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).
Features and Benefits
Excellent retention and peak shape for polar compounds
100% aqueous compatibility
Ultra low bleed, LC-MS compatible
Unique selectivity
Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
