LiChrospher® 100 DIOL (5 µm) HPLC Columns

General description
LiChrospher® DIOL has polar and hydrophobic properties, thus can be used as less polar alternative to LiChrospher Si 60 in normal phase applications or as less hydrophobic alternative to LiChrospher RP-8 in reversed phase applications. LiChrospher DIOL is also suitable for exclusion chromatography.
Application

Chiral and achiral separation of ten flavanones using supercritical fluid chromatography: This study illustrates the application of LiChrospher 100 DIOL in separating flavanones in bee pollen, utilizing both chiral and achiral approaches. The method offers enhanced accuracy and efficiency for analyzing natural products (Ares et al., 2022).

Application of Diol column under adsorption and mixed partition-adsorption normal-phase liquid chromatography mode: This research demonstrates the use of LiChrospher 100 DIOL for the separation of purines and pyrimidines, showcasing its versatility in both adsorption and mixed partition-adsorption chromatographic techniques, which are critical for biochemical analysis (Kazoka, 2008).

Analysis Note
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
stability testing: passes test
Particle Size (d10): 4.8 – 5.5 µm
Particle Size (d50): 6.5 – 7.5 µm
Particle Size (d90): 9.0 – 10.5 µm
Legal Information
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany