Purospher® STAR NH2 (5 µm) HPLC Columns

General description
Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150267]. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART™ cartridges, part number 1.51487.0001 can be used. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC – Method Development Guide
Application
Intended for the separation of alcohols, carboxylic acids, fat-soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water-soluble vitamins.
Features and Benefits
Reproducible results from run-to-run and batch-to-batch
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany