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General description
HPLC column
LiChrosorb® RP-18 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar 125-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.
Application
Simultaneous determination of ampicillin and metampicillin in biological fluids using high-performance liquid chromatography with column switching.: This research introduced an HPLC method with column switching to simultaneously determine ampicillin and metampicillin in biological fluids. The use of LiChrosorb RP-8 (5 micro m) in this method enhanced the separation efficiency, crucial for therapeutic drug monitoring (Lee et al., 1995) (Lee et al., 1995).
Analysis Note
Pressure: ≤ 60 bar
Theoretical Plates (N/m) (Toluene): ≥ 55000
Symmetry (Toluene): 0.8 – 1.4
Capacity factor (Toluene): 1.0 – 2.0
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany
