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General description
Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. The Purospher® STAR RP-18 endcapped, 3 µm LiChroCART™ 55-2 cartridge set contains a Purospher® STAR RP-18 endcapped, 3 µm LiChroCART™ 55-2 cartridge column and a manuCART™ 55 mm cartridge column holder part number 1.50226.0001. LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and has therefore a maximum pressure stability of 250 bar. The column dimension of 55 mm length x 2 mm I.D. is the optimal choice for fast HPLC separations at lower flow rate, allowing significant solvent savings and improved sensitivity. A 2 mm I.D. column enables a more sustainable separations in comparison to 4, 4.6 or 3 mm I.D. column due to the use of significant less solvent for the chromatographic separation.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC – Method Development Guide
Application
Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.
Features and Benefits
Ultra-pure silica (99.999%) for excellent peak symmetry
High separation efficiency
Reproducible results from run-to-run and batch-to-batch
Best all-round performance according to Tanaka
Outstanding pH stability from pH 1.5 to 10.5
No phase collapse when using highly aqueous mobile phases
Good suitability for LC-MS applications
Analysis Note
Pressure: ≤ 55 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 90000
Symmetry (1,2-Benzanthracene): 0.8 – 1.4
Capacity factor (1,2-Benzanthracene): ≤ 10.0
Extent of testing: passes test
Legal Information
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
