Accutase® solution

General description
Accutase® solution (in Dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion- coated plasticware. Accutase is preferred over trypsin for cell detachment as it helps to detach cells from culture vessels with less damage. Hence, Accutase is often used for the treatment of sensitive cells or for analyses of surface markers.
Application
Accutase® solution has been used for dissociation or digestion of nonadherent cells growing in an aggregated cluster, such as brain tumor cells,[1] induced pluripotent stem cells (IPSCs)[2] and glioblastoma cells[3] into single cells.

Suitable for use in preparation of single cell suspension for sequencing.
Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products.
Features and Benefits
Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.
Preparation Note
Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red. Refer to Product Data Sheet on the website for product usage information.
This product contains phenol red. The product is shipped on dry ice and there could be significant CO2 buildup in the package. This CO2 may enter the solution and lower the pH slightly giving a yellow-orange (around pH 6.8) color. The solution should still be good to use as is, or sodium hydroxide may be added to adjust the pH. A fully frozen bottle of Accutase will be yellow in color and change to an orange color as it defrosts. Once unpacked, the product can be refrozen or placed directly into the refrigerator for later use. Do not defrost the product in a warm water bath. Accutase is sensitive to temperatures above 37 °C and will be inactivated after 45 minutes at 37 °C. Defrosting overnight in the refrigerator or in a tub of cold tap water for 1-1.5 hours is recommended. Shake up the bottle of Accutase after defrosting to enable the ingredients to go into solution evenly. These products do not need to be warmed up before being used. Use promptly once removed from the refrigerator, and return to the refrigerator after use.
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.