General description
TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.
Application
TrypZean® Solution, 1× has been used:
for the suspension of pellet during DNA extraction[1]
digestion of cells to single-cell suspension for pharmacotyping of 2D cells[2]
as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium[3]
Features and Benefits
TrypZean® Solution provides the following features and benefits:
Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.

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