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General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Application
RPMI-1640 Medium has been used
to obtain spleen and lymph nodes from mice[1]
for the resuspension of Staphylococcus aureus strains in the phagocytosis assay[2]
in the isolation of hepatitis B surface antigen-specific B-cell clones[3]
Preparation Note
Supplement with 0.3 g/L L-glutamine.
