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General description
Ascentis® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Application
Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employs an Ascentis® Express F5, 5 µm HPLC column for the precise determination of lumacaftor amidst ivacaftor and identifies novel degradation products, showcasing the column′s efficacy in complex mixture analysis (Özcan et al., 2023).
Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: Utilizing the Ascentis® Express F5, 5 µm HPLC column, this research developed stability-indicating methods for tasimelteon, highlighting the column′s robustness in pharmaceutical analysis (Özcan et al., 2020).
Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review explores the advancements in HPLC columns, including the Ascentis® Express F5, emphasizing its role in achieving ultra-fast separations in analytical chemistry (Ali et al., 2012).
Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
