CellASIC ONIX2 Microfluidic System

General description
CellASIC® ONIX2 Microfluidic System is an automated platform for precise manipulation of multiple key cell culture parameters, enabling measurement of cellular responses to pre-programmed media, temperature, and gas environment changes. It provides detailed information about live cell processes with ultra-high quality images and complete environment control. The system utilizes high quality, optically clear microfluidic plates and intuitive software, while integrating with a broad range of inverted microscopes to allow continuous, high magnification observation of live cells—as they react to their environment in time.

Includes:
Controller System: Small footprint, integrated microincubator controller maintains fluid movement, reagent additions, temperature, and gas conditions.
Microfluidic Plates: Application specific plates bring new cell culture capabilities for live cell imaging.
Manifold: Low-profile, high quality, high optical clarity culture chambers and manifold, mount easily on an inverted microscope—without the need for a bulky environmental chamber.
Software: Intuitive software enables quick and easy set-up of detailed protocols for truly automated hands-free cell culture.

Controller System:
Automatically control flow rates, gas and temperature shifts, standing gradients, nutrient/drug additions, and media changes.
Eight critical cell culture parameters can be controlled by the system.
Temperature, oxygen level, growth factors, humidity, pH, osmolality, glucose, ECM and adhesion.

Advantages of the CellASIC® ONIX2 microfluidic plates:
High resolution imaging through optical glass bottom and chamber structures.
Ability to run multiple independent experiments simultaneously.
Continuous access to nutrients and ongoing removal of waste, promoting optimal cell health.

Manifold Advantages:
Manifold seals to the microfluidic plate via “one-touch” vacuum mechanism.
Pressure-driven flow of liquids to the cell chamber provides high precision even at very small volumes.
No contact between the flow system and the biological solutions (on the plate) prevents contamination.
Low-profile manifold is easy to adapt to any inverted microscope stage.

The software offers:
Clearly defined protocols with all experimental parameters saved in one file.
Convenience and reproducibility.
Consistency and reliability.
Error checking, self test, and data logging all make for better troubleshooting and serviceability.
Application
Yeast single cell response.
Bacterial single cell response.
Bacterial biofilm dynamics.
Cell response to hypoxic environments.
Cell response to changing media conditions, drugs, and other stimulants.
Host-pathogen interactions.
Chemotaxis/migration in response to chemogradient.
Setting optimal culture parameters for difficult cell types or long term experiments.
Microscopy of 3D cell culture.
Legal Information
CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany