Superspher® 60 RP-8 (4 µm) HPLC Columns

Application

An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC: This study demonstrates the use of Superspher 60 RP-8 for the high-resolution separation of amino acid derivatives, crucial in biochemical analysis of protein structures. Optimized HPLC protocols improve analytical accuracy and efficiency, beneficial for fields such as analytical chemistry and pharmaceutical analysis (Persson B, Eaker D, 1990).

Analysis Note
Application test: passes test
Theoretical Plates (N/m) (Anthracene): ≥ 105000
stability testing: passes test
selectivity test: passes test
separation factor (Lysine/Digitoxigenine): 1.6 – 2.1
separation factor (Lysine/PHB-propylester): 1.1 – 1.4
separation factor (Naphthalene/Digitoxigenin, selectivity test): 4.5 – 6.5
capacity factor (naphthalene): 17.0 – 22.0
Particle Size (d10): 3.4 – 4.0 µm
Particle Size (d50): 4.6 – 5.3 µm
Particle Size (d90): 6.4 – 7.2 µm
Legal Information
Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany