Thrombin

General description
Thrombin is a Na+ activated allosteric serine protease. It belongs to chymotrypsin family.[1] Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.[1] Thrombin is a coagulation factor IIa.[2] It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg.[3] The enzyme converts fibrinogen to fibrin.[4] The product contains EDTA and additional stabilizing agents.
Application
Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members.[5] It has also been used in platelet spreading on fibrinogen[6][7] and immunostaining.[8] Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.[8][9] Biochem/physiol Actions
Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.[1] Physical form
Lyophilizate
Preparation Note
Storage conditions (working solution): -15 to -25°C

Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Store at 2 to 8 °C. (Store dry!)
The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Other Notes
For life science research only. Not for use in diagnostic procedures.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.