Principle: Qualitative and quantitative real-time PCR for the detection of DNA from gluten-containing cereals.
Kit Format: Real-time PCR kit (64 reactions).
Assay Time: Approximately 100–115 minutes (excluding DNA extraction).
Sample Preparation: DNA is extracted from food samples using a suitable DNA extraction method prior to real-time PCR analysis.
Limit of Detection (LOD): 0.1–1 ppm gluten (depending on the food matrix).
Storage: Store at -15 to -25°C.
Application: Qualitative and quantitative detection of DNA from gluten-containing cereals including wheat, spelt, Khorasan wheat, rye, barley, and triticale in food products.