Sample Preparation
Liquid samples: Dilute the sample and proceed directly with the assay.
Solid samples: Homogenize the sample, extract with the extraction buffer, centrifuge or allow solids to settle, then collect the supernatant for ELISA analysis.
Limit of Detection
0.4 ppm walnut protein.
Recovery
Specificity (Cross-reactivity)
Note:
Almond, buckwheat, and cocoa may produce very low responses (between 0.5 LOQ and 1 LOQ) and, in some cases, may exceed the LOQ.
Limit of Detection (LOD)
0.4 ppm
Limit of Quantification (LOQ)
2 ppm
Quantification Range
2–50 ppm
Calibration Curve
0, 2, 5, 10, 20, and 50 ppm
AlerTox® ELISA Walnut – Walnut Allergen Detection Kit
Peanut (Arachis hypogaea) is a legume belonging to the Fabaceae family (also known as Leguminosae), which includes beans, peas, green beans, alfalfa, and lupins.
Peanut allergy can manifest with a variety of symptoms, ranging from mild oral allergic reactions or urticaria to severe, life-threatening systemic reactions, namely anaphylaxis or bronchial asthma. Peanut-induced anaphylaxis is considered to have the highest fatality rate among all food allergies. Peanut allergy affects more than 0.5% of children in the general population.
AlerTox Sticks Peanut is an ELISA kit for the quantitative detection of peanut antigen in food products, kitchens, and food manufacturing facilities.
Sesame is an herbaceous plant belonging to the Pedaliaceae family. The most common species are Sesamum indicum (white sesame) and Sesamum radiatum (black sesame).
Allergy to sesame seeds and sesame-derived products can manifest with a variety of symptoms, ranging from mild oral allergic reactions or urticaria to severe, life-threatening systemic reactions, namely anaphylaxis or bronchial asthma. Sesame allergy is estimated to affect 0.1%–0.9% of the population and is more common in Asian countries. Sensitive individuals may exhibit cross-reactivity with peanuts, walnuts, hazelnuts, rye, and poppy seeds.
AlerTox Sticks Sesame is a rapid immunochromatographic assay for the qualitative detection of sesame antigen in food products and on environmental surfaces.
Assay Time: 10 minutes.
Almond (Prunus dulcis) belongs to the Rosaceae family (the same family as apples, pears, peaches, cherries, plums, and apricots). However, almond allergy is classified as a tree nut allergy.
Almond allergy can manifest with a variety of symptoms, ranging from mild oral allergic reactions or urticaria to severe, life-threatening systemic reactions, namely anaphylaxis or bronchial asthma.
AlerTox Sticks Almond is an ELISA kit for the semi-quantitative detection of almond antigen in food products, kitchens, and food manufacturing facilities.
Cow’s milk (Bos taurus) and dairy products are a fundamental source of human nutrition. Milk from other species, such as goats, sheep, and buffalo, is also a common source of milk for human consumption in certain geographical regions.
Milk allergy can manifest with a variety of symptoms, ranging from mild oral allergic reactions or skin rashes to severe, life-threatening systemic reactions, namely anaphylaxis or bronchial asthma (IgE- or IgG-mediated responses).
Milk protein allergy can be clinically distinguished from lactose intolerance, which is caused by lactase enzyme deficiency.
Milk allergy is one of the most common food allergies, particularly among infants and young children, affecting 0.5%–5% of the population across different age groups and geographical regions.
AlerTox Beta-Lactoglobulin is a rapid test kit for the quantitative detection of BLG antigen in food products, kitchens, and food manufacturing facilities.
Assay Time: 10 minutes.
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