Sulfonamides Residue ELISA Test Kit

Rapid ELISA Kit for Sulphonamides Residue Testing is a biological immunoassay kit designed for the quantitative analysis of sulphonamides in samples.
Samples: Muscle (tissue), eggs, milk, honey, animal feed.
Testing Time: 75 minutes
Packaging:
HU0050003 – 96 wells (12 strips x 8 wells).

  • Chỉ tiêu thử nghiệm: Sulfonamide
  • Hãng sản xuất: Gold Standard Diagnostics - Mỹ
  • Phương pháp test: Test ELISA
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  • Principle: Competitive ELISA.
  • Plate Format: 96-well plate.
  • Test Samples: Muscle (tissue), eggs, milk, honey, animal feed.
  • Testing Time: 75 minutes.

Sample Preparation:
Muscle, eggs, milk, honey (METHOD I) – Homogenization, solvent extraction, centrifugation, evaporation, reconstitution.
Muscle, eggs, honey (METHOD II) – Sample homogenization, (acid hydrolysis for honey) buffer extraction, centrifugation and filtration.

  • Milk (METHOD II) – Centrifugation and dilution.
  • Honey (METHOD III) – Acid hydrolysis, SPE column purification, elution, evaporation, resuspension.
  • Animal feed: Solvent extraction, dilution.

Limit of Detection:

  • Milk (method I): 0.25 ppb
  • Muscle, eggs, honey (method I): 0.5 ppb
  • Honey (method III): 0.05 ppb
  • Muscle, eggs, honey, milk (method II): 5 ppb
  • Animal feed: 66.6 ppb

Specificity

  • Sulfamerazine >100%
  • Sulfamonomethoxine >100%
  • Sulfadiazine >100%
  • Sulfachloropyridazine >100%
  • Sulfaclozine (sulfachloropyrazine) >100%
  • Sulfamethoxydiazine (sulfamethoxine, sulfameter) >100%
  • Sulfamethoxypyridazine (sulfapyridazine) >100%
  • Sulfadimethoxine >100%
  • Sulfaquinoxaline >100%
  • Sulfathiazole >100%
  • Sulfamethizole >100%
  • Sulfamethazine 100%
  • Sulfamethoxazole 90%
  • Sulfisoxazole 50%
  • Sulfapyridine 36%
  • Sulfadoxine 24%
  • Sulfacetamide 2.5%
  • Sulfaphenazole < 2%
  • Sulfabenzamide < 2%
  • Sulfaguanidine <0.1%

CCb
Muscle (METHOD I): 10 ppb of sulfamethazine; 10 ppb of sulfaquinoxaline
Eggs, milk (METHOD I): 10 ppb of sulfamethazine
Honey (METHOD II): 8 ppb of sulfathiazole
Honey (METHOD III): 5 ppb of sulfamethazine

CV: 6%
Note: Samples evaluated as positive when using the kit must be retested using a confirmatory method.

Sulfonamides Residue ELISA Test Kit
Code: HU0050003
Packaging: 96 wells/kit

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Sulfonamides Residue ELISA Test Kit
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