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General description
Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 3 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART™ 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART™ cartridges, part number 1.51487.0001 can be used.Item 1.50471.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC – Method Development Guide
Application
Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.
Features and Benefits
Ultra-pure silica (99.999%) for excellent peak symmetry
High separation efficiency
Reproducible results from run-to-run and batch-to-batch
Best all-round performance according to Tanaka
Outstanding pH stability from pH 1.5 to 10.5
No phase collapse when using highly aqueous mobile phases
Good suitability for LC-MS applications
Analysis Note
Pressure: ≤ 280 bar
Theoretical Plates (N/m) (Anthracene): ≥ 120000
Symmetry (Anthracene): 0.8 – 1.4
Capacity factor (Anthracene): 4.0 – 6.0
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
