English
General description
COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats [1]. It is an important constituent of type IV collagen in the basement membrane [2]. The COL4A1 gene is located on human chromosome 13q34 [3].
Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.
Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.
Application
Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation.[4] It has also been used as membrane coatings.[1][5]
Biochem/physiol Actions
COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication.[6] This gene is a major constituent required in basal membrane stability.[7] Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.[8]
Features and Benefits
This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
The Collagen Type IV is then purified biochemically.
It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.
