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General description
Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
Application
Minimum essential medium Eagle has been used to:
maintain natural killer (NK) cell lines[1]
culture Leishmania donovani strains[2]
culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles[3]
Preparation Note
Formulated to contain 10.1 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
