HEPES

General description
HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2] It is typically used in cell culture at concentration between 5mM to 30mM.
Useful pH range: 6.8 – 8.2
Application
HEPES has been used:
As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells[3] As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production[4] in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation[5] Other Notes
Easily compare specifications for HEPES products with the HEPES specification table.
This product has been qualified for use in cell and mouse embryo culture.