General description
HPLC cartridge
LiChrospher® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 125-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).
Application
Determination of dopamine, noradrenaline, and adrenaline in Krebs-Henseleit solution by liquid chromatography coupled with tandem mass spectrometry: This study showcases the use of LiChrospher® 100 RP-8 (5 µm) in a LICHROCART™ 125-4 setup for precise neurotransmitter measurement in biological samples, advancing understanding in neurochemistry and cardiovascular research. The column′s selectivity and resolution enhance the detection of these small molecules, pivotal for biochemical assays and physiological studies (Britto-Júnior et al., 2021).
Analysis Note
Pressure: ≤ 45 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 – 1.4
Capacity factor (Toluene): 0.8 – 2.0
Legal Information
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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