English
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC – Method Development Guide
Application
Click postsynthesis of microporous organic network@silica composites: This study presents the creation of novel composites for mixed-mode chromatography, showcasing an innovative approach to chemical separation technologies, which includes hydrophilic interaction liquid chromatography (HILIC) (Sun HF et al., 2023).
Evaluation of Hold-Up Volume Determination Methods: This research evaluates different methods and markers in HILIC, critical for refining analytical methods in complex sample matrices such as pharmaceuticals, enhancing accuracy and reproducibility in chemical analysis (Redon L et al., 2023).
Determination of polyphenolic content in berry juices: Utilizing focusing-modulated comprehensive two-dimensional liquid chromatography, this study highlights the application of HILIC in food analysis, demonstrating its effectiveness in extracting detailed nutritional information (Arena K et al., 2023).
Simple and sensitive determination of sulfites in Chinese herbal teas: This paper employs ultra-high-performance liquid chromatography coupled with mass spectrometry, showing HILIC′s role in ensuring consumer safety by detecting potentially harmful additives in herbal products (Yang L et al., 2022).
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
