EnglishGeneral description
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.
DMEM – high glucose
With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Code: D5796
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
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Sản phẩm tương tự
Poly-ʟ-Ornithine
synthetic, mol wt 30,000-70,000, liquid, 0.01%, suitable for cell culture
Code: P4957
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, pack of 12 ea
Code: S2GPU11RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Dimethyl sulfoxide
Hybri-Max™, 0.2 μm filtered, BioReagent, suitable for hybridoma, ≥99.7%, Ampoule
Code: D2653
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Code: A9647
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Trypsin-EDTA solution
0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Code: T4049
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea
Code: S2GPU05RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture
Code: I9278
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Human Serum
from human male AB plasma, USA origin, sterile-filtered
Code: H4522
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Minimum Essential Medium Eagle
With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: M4655
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Poly-ʟ-Lysine Hydrobromide
synthetic, liquid, 0.01%, suitable for cell culture
Code: P4707
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
