English
General description
McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.
Application
McCoy′s 5A Medium has been used to maintain:
G-361 melanoma cells;
87-MG cells
human colon carcinoma cell line HCT116
Biochem/physiol Actions
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Preparation Note
Supplement with 0.22 g/L L-glutamine.
