English
Application
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
in minimum essential medium (MEM) medium[1]
in the supernatant[2]
in plasma[3]
For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 – 0.56 mM L-glutamate; 0.14 – 1.4 mM L-glutamine.
Biochem/physiol Actions
The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.
