English
General description
Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor.[1] It is majorly expressed in kidney,[2] parotid gland and salivary gland.[3]
Application
Epidermal Growth Factor from murine submaxillary gland has been used
in studying its effects on the development of caustic esophageal stricture in rats[4]
for preparation of MCF-10A cell line[5]
for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)[6]
Biochem/physiol Actions
Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation.[7] EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes.[8] It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia.[1] EGF plays a major role in the maintenance of hair and skin development.[9]
Physical form
Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.
Preparation Note
Purified by gel filtration and ion-exchange chromatography.
Analysis Note
The biological activity is measured in a proliferation assay using BALB/MK cells.
