Transforming Growth Factor-β3 human

General description
TGF-β3 (transforming growth factor-β3) belongs to the TGF β superfamily.[1] The TGFβ3 gene is mapped to human chromosome 14q24.3[2] Biochem/physiol Actions
TGF-β3 (transforming growth factor-β3) regulates lymphocyte proliferation, apoptosis, hematopoiesis and embryogenesis to maintain immune homeostasis.[2] TGF-β plays an important role in cell growth, differentiation, and survival.[3] TGF-β3 specifically promotes chondrogenic differentiation.[3]TGF-β3 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells.[3] TGF-2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils.[4] TGF-β3 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis.[3] TGF-β isoforms is known to participate in wound healing and tissue fibrosis.[5] TGF-β3 is crucial for tissue restoration and scarless tissue repair.[6] Mutation in the TGFβ3 gene is associated with development of non-syndromic cleft palate only (NS CPO), a rare congenital disease.[7] TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.
Physical form
Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5
Preparation Note
HumanKine TGF-β3 is expressed in human HEK 293 cells as a mature, disulfide linked, non- glycosylated homodimer with a predicted molecular mass of 25 kDa.
This product is lyophilized from a solution of 50mM NaOAc, pH 3.7.
Analysis Note
The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc