COLO 699 N

General description
COLO 699 N cells, a non-small cell lung cancer cell line[1] is useful in subcutaneous xenografts studies to mimic in vivo model of human lung cancer.[2] It finds applications in stable isotope-based proteome analysis.[1] Application
COLO 699 N has been used in cytotoxicity studies with Tegaran and 13-methyltetradecanoic acid (13-MTD).[3] Tumourigenicity studies
Biochem/physiol Actions
Derived from the pleural fluid of a 57 year old female with lung cancer. Please note: This cell line was found to be indistinguishable from cell lines RPMI 7932 & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.
Human lung cancer
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.