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General description
Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.[1]
Application
Earle′s Balanced Salt Solution 10x has been used:
as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs[2]
in the swim-up method to prepare human sperm sample[3]
in the steady-state medium to culture bovine primary mammary epithelial cells[1]
Features and Benefits
Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Preparation Note
Supplement with 2.2 g/L sodium bicarbonate at 1×.
