English
General description
Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands[1] containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.
Application
Endothelial cell growth supplement from bovine pituitary has been used:
as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture[2]
as a supplement in medium 199 for endothelial cell culture[3]
as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12[4]
as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture[5]
Biochem/physiol Actions
Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.
