Ethylenediaminetetraacetic acid solution

General description
Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations.[1] Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia.[2] EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques.[3] Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.[4] It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.[5] Application
Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture.[6] It has also been used to detach embryonic stem cells (ESCs) from matrigel[7] and primary human fibroblasts.[8] Physical form
Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium