English
General description
Gamborg′ B-5 medium described by Gamborg, et al. [1] may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.
Application
Gamborg′s B-5 Basal Salt Mixture has been used:
as a constituent during the preparation of TEX buffer[2]
as a media to maintain potato suspension and callus cultures[3]
as a media in the primary-node transformation method[4]
Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Preparation Note
Formulated to contain 3.1 grams of powder per liter of medium.
With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).
Media Formulation
