English
General description
Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Application
Hanks′ Balanced Salt Solution 10x has been used:
as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium[1]
to wash the biopsy specimens of lamina propria lymphocytes (LPLs)[2]
as a wash solution to wash the fish cell line during removal from growth medium[3]
Preparation Note
Supplement with 0.35 g/L sodium bicarbonate at 1X.
