English
Application
Osteopontin from mouse has been used:
to study its role in a mouse model of preterm brain injury[1]
as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques[2]
to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels[3]
Biochem/physiol Actions
Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity.[4] Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to mediate 293 cell adhesion.
