EnglishGeneral description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Preparation Note
Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, suitable for isotope labeling for cell culture applications
Code: R1780
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
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Sản phẩm tương tự
Dimethyl sulfoxide
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Code: D2438
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
USDA approved, sterile-filtered, suitable for cell culture
Code: F0926
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
CryoStor® cell cryopreservation media
CS10
Code: C2874
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Fibronectin
from bovine plasma, liquid, suitable for cell culture
Code: F1141
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea
Code: S2GPU05RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Fetal Bovine Serum
non-USA origin, sterile-filtered, suitable for cell culture
Code: F7524
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Human Serum
from human male AB plasma, USA origin, sterile-filtered
Code: H4522
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Steriflip® Vacuum Tube Top Filter
pore size 0.22 μm, polyethersulfone membrane, funnel capacity 50 mL, pack of 25 ea
Code: SCGP00525
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Bovine Serum Albumin
lyophilized powder, ≥96% (agarose gel electrophoresis)
Code: A2153
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Code: A9647
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
