EnglishGeneral description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.
Application
RPMI-1640 Medium has been used:
to culture human lymphoblastoid cell line cells[1]
prostate cancer cells[2]
monocytes from human blood[3]
RPMI-1640 Medium
HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Code: R5886100 mL R5886-100ML
500 mL R5886-500ML
6 x 500 mL R5886-6X500ML
24 x 500 mL R5886-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
| Hãng sản xuất |
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Sản phẩm tương tự
Bovine Serum Albumin
heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
Code: A703010 g A7030-10G
50 g A7030-50G
100 g A7030-100G
500 g A7030-500G
1 kg A7030-1KG
5 kg A7030-5KG
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
USDA approved, sterile-filtered, suitable for cell culture
Code: F0926
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
≥99.5% (GC), suitable for plant cell culture
Code: D4540
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Code: A9647
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
DMEM – high glucose
With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Code: D5796
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture
Code: I9278
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
DMEM – high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Code: D6429
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Trypsin-EDTA solution
0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Code: T4049
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea
Code: S2GPU05RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Dulbecco′s Phosphate Buffered Saline
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Code: D8537100 mL D8537-100ML
500 mL D8537-500ML
1 L D8537-1L
6 x 500 mL D8537-6X500ML
6 x 1 L D8537-6X1L
24 x 500 mL D8537-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
