English
General description
Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.[1]
Application
Transferrin human has been used:
to incubate human proximal tubule cells (HKC) – 8 cells along with IgG for the examination of albumin specificity[2]
as a supplement in serum-containing/devoid medium for human thyroid cell culture[3]
as a target glycoprotein to develop an in-gel hydrazinolysis method[4]
Biochem/physiol Actions
Transferrin mediates iron transportation from plasma to cells.[1] The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin.[1] The transferrin binds to specific cell surface receptors and are internalized by endocytosis.[5] Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded.[5][6] Transferrin is known to deliver iron to cells in a physiologically safe and effective form.
Preparation Note
Heat treated at 60 °C for ten hours.
Analysis Note
Purity by agarose electrophoresis.
Other Notes
Partially iron-saturated
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
