EnglishApplication
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.
Preparation Note
Formulated to contain 13.5 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate
Dulbecco′s Modified Eagle′s Medium – high glucose
With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
Code: D777710 L D7777-10L
10 x 1 L D7777-10X1L
50 L D7777-50L
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
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Sản phẩm tương tự
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Code: A9647
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Code: D2650
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Poly-ʟ-Ornithine
synthetic, mol wt 30,000-70,000, liquid, 0.01%, suitable for cell culture
Code: P4957
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Code: D2438
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
D-(+)-Glucose
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Code: G7021
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
CryoStor® cell cryopreservation media
CS10
Code: C2874
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Poly-ʟ-Lysine Hydrobromide
synthetic, liquid, 0.01%, suitable for cell culture
Code: P4707
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Steriflip® Vacuum Tube Top Filter
pore size 0.22 μm, polyethersulfone membrane, funnel capacity 50 mL, pack of 25 ea
Code: SCGP00525
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
DMEM – high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Code: D6429
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Code: F2442
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
