EnglishApplication
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.
Preparation Note
Formulated to contain 13.5 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate
Dulbecco′s Modified Eagle′s Medium – high glucose
With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
Code: D777710 L D7777-10L
10 x 1 L D7777-10X1L
50 L D7777-50L
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
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Sản phẩm tương tự
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea
Code: S2GPU05RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: R8758100 mL R8758-100ML
500 mL R8758-500ML
1 L R8758-1L
6 x 500 mL R8758-6X500ML
6 x 1 L R8758-6X1L
24 x 500 mL R8758-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
Hybri-Max™, 0.2 μm filtered, BioReagent, suitable for hybridoma, ≥99.7%, Ampoule
Code: D2653
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Serum Albumin
heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
Code: A703010 g A7030-10G
50 g A7030-50G
100 g A7030-100G
500 g A7030-500G
1 kg A7030-1KG
5 kg A7030-5KG
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
USDA approved, sterile-filtered, suitable for cell culture
Code: F0926
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Accutase® solution
sterile-filtered, suitable for cell culture
Code: A6964
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dulbecco′s Phosphate Buffered Saline
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Code: D8537100 mL D8537-100ML
500 mL D8537-500ML
1 L D8537-1L
6 x 500 mL D8537-6X500ML
6 x 1 L D8537-6X1L
24 x 500 mL D8537-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Hyaluronidase from bovine testes
Type I-S, lyophilized powder, 400-1000 units/mg solid
Code: H3506
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture
Code: I9278
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Minimum Essential Medium Eagle
With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: M4655
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
