EnglishGeneral description
Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium.[1] It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.[2]
Application
GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells[3] and selective medium for NS0 myeloma cells.[4]
Packaging
Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0
Preparation Note
One mL will supplement 50 mL of cell culture medium.
GSEM Supplement
liquid, suitable for cell culture,
Code: G9785
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
| Hãng sản xuất |
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Sản phẩm tương tự
Trypsin-EDTA solution
0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Code: T4049
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dulbecco′s Phosphate Buffered Saline
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Code: D8537100 mL D8537-100ML
500 mL D8537-500ML
1 L D8537-1L
6 x 500 mL D8537-6X500ML
6 x 1 L D8537-6X1L
24 x 500 mL D8537-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture
Code: I9278
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea
Code: S2GPU05RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Code: D2650
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
CryoStor® cell cryopreservation media
CS10
Code: C2874
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
DMEM – high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Code: D6429
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, pack of 12 ea
Code: S2GPU11RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Dimethyl sulfoxide
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Code: D2438
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: R8758100 mL R8758-100ML
500 mL R8758-500ML
1 L R8758-1L
6 x 500 mL R8758-6X500ML
6 x 1 L R8758-6X1L
24 x 500 mL R8758-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
