3dGRO® L-WRN Conditioned Media Supplement

Application
Appearance (color): clear, red liquid
Endotoxin: <2 EU/mL Sterility Tested: No Growth/Pass Media are tested for function in supporting growth of human intestinal organoids Media are tested for levels of growth factors by ELISA The 3dGRO® L-WRN Conditioned Media Supplement is a formulation based on DMEM/F-12 PLUS medium containing the 3 growth factors Wnt3a, R-spondin-3, and Noggin. These factors act upon the Wnt signaling pathway, which plays a critical role in sustaining the proliferation of LGR5+ crypt stem cells within organoids. Wnt activation is thus essential for in vitro growth of human gut organoids. L-WRN conditioned medium is widely used as a component of the culture medium for supporting the growth of human intestinal organoids including the 3dGRO® gastrointestinal organoid biobank (SCC310-SCC367). Applications for L-WRN conditioned medium include but are not limited to the expansion of human intestinal gut organoids and other epithelial organoid cell types. Source The 3dGRO® L-WRN Conditioned Media Supplement is derived from the Mus musculus L-M(TK-) cell line secreting mouse Wnt3a, R-spondin-3 and Noggin. L-WRN Conditioned Medium may contain trace amounts of mouse DNA. Features and Benefits L-WRN conditioned media supplement containing the 3 growth factors Wnt3a (W), R-spondin-3 (R), and Noggin (N) for organoid cultures. Preparation Note The 3dGRO® L-WRN Conditioned Media Supplement should be stored at -20°C. When ready to use, thaw the L-WRN Conditioned Medium overnight at 2-8°C in the dark. Thawed medium is good for two weeks when stored at 2-8°C. If media will not be used within two weeks, aliquot unused media and store at -20°C. After thawing refrozen aliquots, do not re-freeze media. Legal Information 3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.