English
General description
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.[1][2]
Application
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
to block tissue sections of placenta in immunohistochemical (IHC) analysis[3]
as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation[4]
as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo[5]
Biochem/physiol Actions
Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs.[1][6] It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7]
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
