English
General description
Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each.[1][2] This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.[1][2]
Application
Albumin fraction V has been used:
as a component of perfusion buffer for perfusion protocol for rodent organ perfusion [3]
as a blocking agent in western blot analysis[4]
as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays[5]
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the chief component of cell culture media[6] and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7] It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids.[6][1]
Analysis Note
Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 – 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
