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General description
High purity plasma protein prepared under non-denaturing conditions.
Human serum albumin (HSA) is the most abundant protein present in the plasma.[1] It is a monomeric multi-domain macromolecule comprising three homologous domains, numbered I, II, and III.[1][2] Each domain consists of subdomains A and B with common structural motifs. The HSA gene is mapped to the human chromosome 4q13.3.[2]
Application
Albumin, human serum, non-denatured has been used:
to determine the contribution of albumin protein complexes to esterase activity in saliva[3],
in tris buffer for monomer degradation studies and in protein adhesion assay[4]
to study the effect of Cu2+ and Zn2+ ions on human serum albumin interaction with plasma unsaturated fatty acids[5]
Biochem/physiol Actions
Human serum albumin (HSA) functions as a carrier for fatty acids, steroids, and thyroid hormones.[6] It is used as a supplement in cell culture medium to produce vaccines and pharmaceuticals.[6] HSA is a potential biomarker for various diseases, like cancer, rheumatoid arthritis, ischemia, and post-menopausal obesity. It is also used to treat surgical blood loss, trauma, hemorrhage, and cardiopulmonary bypass. HSA is implicated in biological applications such as implantable biomaterials, surgical adhesives and sealants, and bio chromatography.[1]
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Travis, J., and Pannell, R. 1973. Clin. Chem. Acta49, 49.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)
