CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling

Application
CellVue Claret kit fluoresces in the far red and may be used for general cell membrane labeling including in vitro cell labeling, in vitro lymphocyte proliferation studies,† in vivo cytotoxicity assays,† and also as a second color for monitoring cell-cell membrane transfer.†
CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling has been used:
to label monocyte-derived macrophages (MDMs) for antibody-dependent cellular phagocytosis assay[1] in labeling of Jurkat cells after the induction of apoptosi[2] in labeling of cells in mice for assaying the retention of tumor cells in lung[3]

Biochem/physiol Actions
The CellVue Claret Fluorescent Cell Linker kit uses a proprietary membrane labeling technology to stably incorporate a far red fluorescent dye with long aliphatic tails (CellVue Claret) into lipid regions of the cell membrane.†
Packaging
This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.
Other Notes
For additional technical details on Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit PKH and CellVue®; Claret Fluorescent Cell Linker Dyes.
Legal Information
Distributed for Phanos Technologies, Inc.
Patent Information
CellVue is a registered trademark of Phanos Technologies