Dimethyl sulfoxide

Application
Dimethyl sulfoxide has been used:
for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments[1] as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells[2] for the suspension and separation of buccal cells[3] a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.
Biochem/physiol Actions
Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant.[4] It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.[5] Other Notes
For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Disclaimer
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.