IFN-γ human

General description
IFN-γ (interferon γ) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells.[1][2][3] IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells.[4] The gene is mapped to human chromosome 12q15.[5] Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7kDa protein containing 143 amino acid residues.
Application
IFN-γ (interferon γ)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.[6][7] Biochem/physiol Actions
IFN-γ (interferon γ) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response.[8][9] It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines.[10] Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils.[11][12] It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis.[3] IFN-γ is also involved in Hepatitis E virus disease pathogenesis.[13] Physical form
Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium phosphate, 2% trehalose, 4% mannitol, 0.1% Tween 80, at pH 6.5 with a protein concentration of 0.5 mg/mL.
Preparation Note
Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
Other Notes
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ