IFN-alpha 1 human

General description
IFN-α1 (interferon α 1) is a type 1 interferon.[1] Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes.[2] The IFN-α1 gene is mapped to human chromosome 9p21.3.[3] Application
IFN-alpha 1 human has been used:
to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection[4] as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography[5] to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation[6] as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response[7] Biochem/physiol Actions
IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity.[8] IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins.[9] IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma.[10] The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus.[11] Physical form
Lyophilized without any additives.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.